Professor
Research interests：Synthetic biology, Metabolic engineering，Enzyme engineering
Email：shuangli@scut.edu.cn
13826293239
Education
2001 B. S., Chemical Engineering, Tsinghua University, Beijing,
2006 Ph.D., Biochemical Engineering, Tsinghua University, Beijing,
Professional Experience
2015.9-present Professor, School of Bioscience and Bioengineering, South China University of Technology
2010.9-2015.8Associate Professor, School of Bioscience and Bioengineering, South China University of Technology
2012.9-2013.9Visiting scholar, Chemical and Biomolecular Engineering, UCLA
2007.8-2010.8Assistant Professor, School of Bioscience and Bioengineering, South China University of Technology
2006.8-2007.7Manager of R&D Centre, Guangzhou Baidi Bio-technology Co., Ltd.
Research Interests
To development the basic methods and to build the key technical platform on synthetic biology. Focusing on the micro-production of energy, fine chemicals and human health related products.
(1) To construct cell factory to produce targets based on the techniques or theory of synthetic biology.
(2) To redirect the metabolic pathway to increase the productivity of targets.
(3) Enzyme engineering.
Honors and Awards
2012 Pearl River New-Star of Science &Technology supported by Guangzhou City 2011 Young Scientists Prize (Top 3), TWAS-ROESEAP Symposium on Industrial Biotechnology, in Beijing
Main fundings
[1]农药残留遗传毒性物质的快速筛查技术开发广州市产学研协同创新重大专项 2017/05-2020/04 主持
[2]嗜热厌氧杆菌碳代谢物阻遏机制解析及其解除方案构建研究广东省自然科学基金 2014/10-2017/10 主持
[3]原位分离耦合高密度发酵生产生物质乙醇的工艺开发广东省科技厅国际合作项目 2015/01-2016/12 主持
[4]CcpA介导的嗜热氧杆菌碳代谢物阻遏机制解析及解除研究国家自然科学基金(面上项目) 2013/01-2016/12 主持
[5]农林废弃物生产乙醇和H2的代谢调控及发酵工艺研究广州市珠江科技新星专项 2012/07-2015/06 主持
[6]关于改善甘油脱水酶机理性失活的研究国家自然科学基金(青年基金) 2008/01-2011/12，主持
[7]生物催化剂的发现及进化机制解析国家重点基础研究发展计划资助(973计划)子课题2009/01-2013/12 主持
[8]酶制剂及生物催化关键技术产业化国家十一五支撑计划 2009/01-2010/12 主持
[9]产圆柚酮的酿酒酵母细胞工厂产能优化广东省科技发展专项 2017/07-2010/06 主持
[10]糖转运蛋白对嗜热菌利用木糖发酵产氢的机理研究广东省自然科学基金 2011/10-2013/10 主持
Selected Publications
1.Zhu M, Lu Y, Wang J, Li S*, Wang X, Carbon catabolite repression and the related genes of ccpA, ptsH and hprK in Thermoanaerobacterium aotearoense. PLoS ONE, 2015, 10:e0142121
2.Yang X, Zhu M, Huang X, Lin CSK, Wang J, Li S*, Valorisation of mixed bakery waste in non-sterilized fermentation for l-lactic acid production by an evolved Thermoanaerobacterium sp. strain. Bioresource Technol, 2015, 198:47-54. SCI 1区, IF=5.04
3.Huang X, Li Z, Du C, Wang J, Li S*, Improved expression and characterization of a multi-domain xylanase from Thermoanaerobacterium aotearoense SCUT27 in Bacillus subtilis. J Arg Food Chem, 2015, 63:6430-6439. SCI 1区
4.Yang F, Yang X, Li Z, Du C, Wang J, Li S*, Overexpression and characterization of a glucose-tolerant β-glucosidase from T. aotearoense with high specific activity for cellobiose. Appl Microbiol Biotechnol, 2015, 99:8903-8915. SCI 2区
5.Yang X, Huang A, Peng J, Wang J, Wang X, Lin Z*, Li S*, Self-assembly amphipathic peptides induce active enzyme aggregation that dramatically increases the operational stability of nitrilase, RSC Advances, 2014, 4: 60675-60684, SCI 3区
6.Lai Z, Zhu M, Yang X, Wang J, Li S* Optimization of key factors affecting hydrogen production from sugarcane bagasse by a thermophilic anaerobic pure culture. Biotechnol Biofuels 2014, 7:119. SCI 1区, IF=6.44
7.Yang X, Lai Z, Lai C, Zhu M, Li S*, Wang J*, Wang X: Efficient production of l-lactic acid by an engineered Thermoanaerobacterium aotearoense with broad substrate specificity. Biotechnol Biofuels 2013, 6:124. SCI 1区, IF=6.44
8.Xu W, Chooi YH, Choi JW,Li S, Vederas JC, Da Silva NA, Tang Y: LovG: the thioesterase required for dihydromonacolin L release and lovastatin nonaketide synthase turnover in lovastatin biosynthesis. Angew Chem Int Ed Engl 2013, 52:6472-6475. SCI 1区, IF=11.71
9.Li S, Yang X, Yang S, Zhu M, Wang X: Technology Prospecting on Enzymes: Application, Marketing and Engineering. Comput Struct Biotechnol J 2012, 2:e201209017. (Most cited article, top 3 in the journal)
10.Li S, Lai C, Cai Y, Yang X, Yang S, Zhu M, Wang J, Wang X: High efficiency hydrogen production from glucose/xylose by the ldh-deleted Thermoanaerobacterium strain. Bioresour Technol 2010, 101:8718-8724. SCI 1区IF=5.04
Book Chapter
1.Li S, Yang X (2016) Biofuel production from food wastes. In: Luque R, Lin CSK, Wilson K, Clark J (eds) Handbook of Biofuels Production. 2ed. Woodhead Publishing, Oxford, UK
2.Lin Z, Li S, Chen Y (2009) Identification of Viral Peptide Fragments for Vaccine Development. In: Hicks BW (ed) Methods in Molecular Biology. Viral Applications of Green Fluorescent Protein vol 515. Humana Press, New York, pp 261-274
Issued patents
[1]Li Shuang, Lai Zhicheng, Wang jufang. A Thermoanaerobacterium and the ethanol production method, 201410453513.8
[2]Li Shuang, Yang Xiaofeng, Lin Zhanglin, Wang Jufang, Wang Xiaoning, Nitrilase immobilization based on self-assembling peptide induced aggregates, 201410019182.7.
[3]Wang Jufang, Li Lin, Li Shuang, Yu Pingru, Butanol production by coculture of two strains, CN201210434676.2.
[4]Wang Jufang, Li Lin, Li Shuang, Yu Pingru, Instruments for the butanol production by by coculture of two strains, CN201220575734.9.
[5]Zhanglin Lin, Shuang Li, Zhen Cai, A method for preparation of functional peptide fragments. CN 200410000143.9
[6]Zhanglin Lin, Shuang Li, Lihua Xu, A High-throughput screening system dependent on inducible cell lysis, CN 200610078952.0
[7]Zhanglin Lin, Lihua Xu, Shuang Li, Chuan Ren, Methods and applications of Escherichia coli Cell Autolysis CN 200610078951.6
Meetings
[1]Li S (2017) Hydrogen production, Adaptive evolution and Transcriptome analysis of Metabolic Engineered Thermoanaerobacterium sp. strain. At the 2nd International Conference on Biological Waste as Resource (BWR2017) Hong Kong. (Invited speaker, Member of Scientific Committee, Chair)
[2]Li S, Yang X, Huang A (2016) Self-assembly amphipathic peptides induce active enzyme aggregation that dramatically increases the operational stability of nitrilase. At the 8th Global Chinese Chemical Engineering Symposium, Singapore. (Poster)
[3]Li S, Zhu M, Yang X (2016) Non-sterilized fermentation for ethanol or L-Lactic Acid from waste substrate by Metabolic Engineering of Thermoanaerobacterium sp. strain. At the 11th Metabolic Engineering Conference, Kobe, Japan (Poster)
[4]Li S, Huang A, Yang X (2015) Characteristic Improvement of the Active Enzyme Aggregation Induced by Self-Assembly Peptides. At the 6th Annual World Congress of Enzymes and Biocatalysts, Nanjing, China. (co-Chair, Oral)
[5]Li S (2014a) Catalytic properties improvements of Alcaligenes faecalis nitrilase by self-assembly induced aggregation. Paper presented at the 16th European Congress on Biotechnology Edinburgh, Scotland(Oral)
[6]Li S (2014b) Non-sterilized production of Ethanol or Optically pure L-Lactic Acid from Cellulosic Substrate by Metabolic Directing of Thermoanaerobacterium. At the 1st Annual International Symposium of Industrial Microbiology Dalian, China. (Oral)
[7]Li S (2013) Catalysis Characteristic Research of Self-assembly Peptides Induced Nitrilase Aggregates. At the 5th Symposium on Enzymes and Biocatalysis, Dalian, China. (Oral)
[8]Li S (2012) Metabolic Control of Xylose-Utilizing Thermoanaerobacterium to Produce Chemicals. At the 5th Annual World Congress of Industrial Biotechnology, Theme: Bio-economy in Synthetic Bio-era, Xi'an, China. (Oral)
