Abstract：Regeneration  of zwitterionic surfaces is a promising strategy to combat biofouling.  In this study, we have prepared degradable hyperbranched anti-biofouling  polymers of methyl methacrylate, tertiary carboxybetaine ester (TCB),  and divinyl monomers  via reversible addition-fragmentation chain transfer (RAFT)  polymerization. The degradation rate of such polymers is a function of  the content or type of divinyl monomers. As the content of methacrylic  anhydride (MAAH) increases, the degradation rate of the polymer becomes  faster; whereas, a change of the divinyl monomers causes the degradation  rate to follow a descending order: N,N′-adipic bis(diacetone acrylamide hydrazone) (DAA2H) > MAAH > ethylene glycol  dimethacrylate (EGDMA). Antibacterial and anti-diatom assays show that  the coatings inhibit the adhesion of the marine bacteria Pseudomonas sp. and the diatom Navicula incerta. The polymeric coating is expected to be applied in marine anti-biofouling.