Research interest:Protein Science and Protein Engineering
E-mail: bimayikobe@scut.edu.cn
Education& Occupation
2004/09-2007/06 Master (Biochemistry&Molecular Biology), Hunan University
2007/09-2012/06 Ph.D. (Microbiology), South China University of Technology& Goethe-University, Frankfurt am Main, Germany
2012/12-2016/08 Lecturer, School of Bioscience and Bioengineering, South China University of Technology
Since 2016/09 Associate Professor, School of Bioscience and Bioengineering, South China University of Technology
Research Experience and Interests
The main focus of our research contains: 1) The establishment and development of cell free reaction systemfor efficient expression, purification, function characterization, ligand screening and structure analysis of important drug targeted membrane proteins; 2) The designing of new antimicrobial peptides forindustrial production as well as development and preparation of high efficient, broad-spectrum and non-toxic antibacterial medicines; 3) The development of foods and products for removing heavy metals and free radical, anti-aging and anti-radiation; 4) The optimization of expression system from Escherichia coli, Pichia yeast, Bacillus subtilis and Lactococcuslactis for efficient expression of recombinant proteins; 5) The modification of thermophilic anaerobic bacteria and high density-fermentation to producebiofuel ethanol. Be supported by more than 10 projects and grants from government and industrial companies. Published 16 research papers and applied 7 patents.
Research Projects:
[1]. National Natural Science Foundation of China (Principal Investigator): Study on the in vitro synthetic pathway of bacterial peptidoglycan monomer, 2014.01-2016.12
[2]. Natural Science Foundation of Guangdong (Principal Investigator): Functional and three-dimensional structure analysis of the membrane protein, MraY translocation enzyme in biosynthesis pathway of bacterial cell wall, 2014.10-2017.09
[3].Research Fund for the Doctoral Program of Higher Education of China (Principal Investigator): Study on the in vitrobiosynthesis of peptidoglycan monomer, 2014.01-2016.12
[4].The Fundamental Research Funds for the Central Universities (Principal Investigator): Study on the function and three-dimensional structure of the novel antibiotic target, MraY translocation enzyme inMycobacterium tuberculosis, 2015.07-2017.07
[5]. The Fundamental Research Funds for the Central Universities (Principal Investigator): In vitro construction of bacterial cell wall peptidoglycan monomer synthesis pathway and its application in antibacterial drug research and development, 2013.01-2015.12
[6]. The Innovation Projects of General Higher Education of Guangdong (Principal Investigator): Molecular modification and functional characterization of protein E in PhiX174, a key factor to prepare bacterial ghost,2017.01-2018.12
[7]. Science and technology project from enterprise (Principal Investigator): The key technology to applycell wall polysaccharide in Saccharomyces cerevisiae, 2017.02-2018.01
SelectedPublications:
(*: Corresponding author; #: Co-first author)
[1]. Erik Henrich, Yi Ma*, Ina Engels, Daniela Münch, Christian Otten, Tanja Schneider, Beate Henrichfreise, Hans-Georg Sahl, Volker Dötsch and Frank Bernhard.Lipid Requirements for the Enzymatic Activity of MraY Translocases and in Vitro Reconstitution of the Lipid Ⅱ Synthesis Pathway.Journal of Biological Chemistry, 291(5): 2535-2546, 2016.
[2]. Yi Ma,Daniela Münch, Tanja Schneider, Hans-Georg Sahl, Ahmed Bouhss, UmeshGhoshdastider, Jufang Wang, Volker Dötsch, Xiaoning Wang and Frank Bernhard.Preparative Scale Cell-free Production and Quality Optimization of MraY Homologues in Different Expression Modes. The Journal of Biological Chemistry, 286 (45): 38844-38853, 2011.
[3]. Meng Wang, Minhua Huang, Huawei Gu, Shan Li, Yi Ma*and Jufang Wang. Mutational Analysis to Identify the Residues Essential for the Acetyltransferase Activity of GlmU in Bacillus subtilis. RSC Advances, 7(23): 13858-13867, 2017.
[4]. Yi Ma, Chao Ni, Emmanuel E. Dzakah, Haiying Wang, Keren Kang, Shixing Tang, Jihua Wang, and Jufang Wang. Development of Monoclonal Antibodies against HIV-1 p24 Protein and its Application in Colloidal Gold Immunochromatographic Assay for HIV-1 Detection. BioMed Research International, 6743904, 6 pages, 2016.
[5]. Yi Ma, Jieying Yu, Jinglian Lin, ShaominWu, Shan Li, and JufangWang. High Efficient Expression, Purification and Functional Identification of Native Human Epidermal Growth Factor in Escherichia coli. BioMed Research International, 3758941, 7 pages, 2016.
[6]. Yi Ma, Beilei Zhang, Meng Wang, Yanghui Ou, Jufang Wang, and Shan Li. Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from Geobacillus stearothermophilus by Site-Directed Mutagenesis at the Active Site. BioMed Research International, 2906484, 8 pages, 2016.
[7]. Yi Ma, Umesh Ghoshdastider, Jiufang Wang, Volker Dötsch, Frank Bernhard and Xiaoning Wang. Cell-free Expression of Human Glucosamine 6-phosphate N-acetyltransferase (HsGNA1) for Inhibitor Screening. Protein Expression and Purification, 86 (2):120-126, 2012.
[8]. Wei Ye#, Yi Ma#, Haiying Wang, Xiaochun Luo, Weimin Zhang, Jufang Wang*, Xiaoning Wang. A New Strategy for Recovery of Bioactive Peptide Employing Glutamate-specific Endopeptidase from Bacillus licheniformis. Enzyme and Microbial Technology. 54: 25-31, 2014.
[9]. Ji Liu#, Yi Ma#, Chun-Li Sun, Shan Li, and Ju-Fang Wang. High Mobility Group Box1 Protein is Involved in Endoplasmic Reticulum Stress Induced by Clostridium difficile Toxin A. BioMed Research International, , 4130834, 7 pages, 2016.
[10]. Yanfang Su, Jing Yang, Meng Wang, Shaomin Wu, Yi Ma*, Shan Li, and Jufang Wang. Efficient and Functional Expression of cA-GFP Fusion Protein in Escherichia coli. Modern Food Science and Technology, 31(12): 128-133, 2015
Patents:
[1]. Jufang Wang, Wei Ye, Haiying Wang, Yi Ma,Pingru Yu, Xiaoning Wang.A method of preparation of mature human beta defensing-2.(ZL 201310008341.9)
[2]. Jufang Wang, Haiying Wang、Fatima、Yi Ma, Xiaoning Wang.A method of preparation and application of heavy chain single domain antibody from type II dengue fever NS1.(ZL201310303381.6)
[3]. Jufang Wang, Yi Ma, Shan Li, Shaomin Wu, Jieying Yu.A method of functional expression of human epidermal cell growth factor in Escherichia coli. (201610586211.7)
[4]. Jufang Wang, Yi Ma, Jing Yang，Shan Li. A method for producing recombinant human type I collagen by immobilized fermentation. (201510535383.7)
[5]. Jufang Wang, Yi Ma, Shan Li, Yanfang Su. A method forpreparation and application of human metallothionein type I anchored on cell surface of food grade Lactococuslactis. (201510155501.1)
[6]. Jufang Wang, Qi Zhong, Yi Ma, Tingting Liu, Haiying Wang. A method of optical analysis detection of CHO cell proliferation. (201410788129.3)
[7]. Jufang Wang, Ping Luo, Yi Ma, Shan Li. A Saccharomyces cerevisiae strain with high tolerance of furfural.(201510854364.0)
