Lizhen Huang
E-mail: huanglzh@scut.edu.cn.
Phone: 13631406992
Education and research experience
Education experience
2007-2010 Southern Medical University PH.D.
2004-2007 Southern Medical University MS
2000-2004 Jiangxi Agricultural University BS
Research & work experience
2005 - 2007，Developing HBV transgenic mice and doing anti-HBV drug screening in the Transgenic lab of the PLA 458 hospital.
2008 – 2010，Doing research on transgenic and gene knockout pig as human disease animal models in South China Stem Cells and Regenerative Medicine Institute of Chinese Academy of Science
2011 – now， Research on genetically modified cell models and animal models in School of Bioscience and Bioengineering,South China University of Technology
Research interest and achievement
-Research Interests
High and stable expression: construction of high expression mammalian system, gene knockin in safe-harbor position, in step gene reporter system.
Gene knockout or gene mutation: gene knockout or mutation in cells or animals with TALEN, CRISPR/CAS9 technologies.
-Achievement
I have implemented 1 project from Natural Science Foundation, 1 project from Guangdong science and technology project, 2 projects from the Fundamental Research Funds for the Central Universities. I won the 1st class Guangdong province award of science & technology progression and the 1st class Guangzhou city award of science & technology progression. About 5 SCI paper were published and 2 patents were applied in recent 5 years.
The main research projects
(1)Establishment of high sensitive hepatocyte model for drug metabolism by piggyBactransposition , supported by the National Natural Science Foundation of China (2012.1 - 2015.12 ), Host；
(2) Development and application of primers for SNP marker screening in mammalian experimental animals, Supported by Science and Technology Planning Project of Guangdong Province, China，2015.1-2016.12，Host；
(3) The study of hepatotoxicity mechanism induced by diclofenac with humanized mice, supported by the Fundamental Research Funds for the Central Universities, 2013.1-2015.12，Host;
The establishment of minipig CYP3A in vitro expression system and the comparation of its metabolic representation, supported by the Fundamental Research Funds for the Central Universities, 2011.12-2012.12，Host.
Papers
[1] Huang L, Zou S, Deng J, Dai T, Jiang J, Jia Y, Dai R, Xie S. Development of an optimized cytotoxicity assay system for CYP3A4-mediated metabolic activation via modified piggyBac transposition. Toxicol In Vitro. 2015 Dec 17;32:132-137. doi: 10.1016/j.tiv.2015.12.008.
[2] Xie S, Wang H, Liu Z, Dai R, Huang L. Fluorescent metal–organic framework based on pyrene chromophore for sensing of nitrobenzene. RSC Adv., 2015,5, 7121-7124.
[3] Xie S, Jia Y, Liu A, Dai R, Huang L. Hypaconitine-induced QT prolongation Mediated through inhibition of KCNH2 (hERG) potassium Channels in conscious Dogs. J Ethnopharmacol. 2015 Mar 20. pii: S0378-8741(15)00167-1
[4] Lizhen Huang, Nana Fan, Jie Cai, Dongshan Yang, Bentian Zhao, Zhen Ouyang, Weiwang Gu, and Liangxue Lai. Establishment of a Porcine Oct-4 Promoter-Driven EGFP Reporter System for Monitoring Pluripotency of Porcine Stem Cells. Cellular Reprogramming, 2011, 13(2): 93-98.
[5] Jingwei Jiang, Lizhen Huang (co-first authors), Xiaohua Liang, Xinlin Zhou, Ruofan Huang, Zhaohui Chu, Qiong Zhang. Gefitinib versus docetaxel in previously treated advanced non-small-cell lung cancer: a meta-analysis of randomized controlled trials, Acta Oncol. 2011 May;50(4):582-8.
Patents
[1] Development and application of primers for SNP marker screening in mammalian experimental animals （CN201510234108.1）
Inventor: Du Hongli, Wang Jufang, Liu Minyu, Zhao Yi, Huang Lizhen
[2] The coexpression system of human CYP3A5 and NADPH（CN201310024136.1）
Inventor: Dai Renke, Zhang Wei,Huang Lizhen, Deng Jifeng, Wang Zhenyu, Xie Shuilin
