Tel：13249649282，Email：lishan@scut.edu.cn
Research interests: biochemistry and molecular biology, protein engineering,Monoclonal antibodies, screening of antibodies and immunotherapy
Education and Professional Profile
2014-present Associate Professor, School of Bioscience and Bioengineering, South China University of Technology
2013-2014 Lecturer, School of Bioscience and Bioengineering, South China University of Technology
2006-2012 Ph.D. of Fermentation engineering, South China University of Technology
2002-2006 Bachelor of Bioengineering, South China University of Technology
Research interests and major achievements
Our research interests concern the Enzymology and Enzyme engineering and aim to excavate and modify the enzyme molecular, highly express enzyme proteins, regulate microbial metabolism and synthesis high-value natural products. Analyze the expression mechanism of heterologous proteins in Pichia pastoris through transcriptomics and proteomics techniques based on high throughput sequencing. The enzyme proteins were efficiently expressed by expression elements optimization (promoter, secretory signal peptide, untranslated region) and secretory pathways rational regulation (translation, Folding, transport) and used in sweetener modification, aromatic ester synthesis and other fields.
Representative research projects
1. Functional identification of the transcription factor Fhl1p and the mechanism of enhancement of heterogeneous protein expression in Pichia pastoris, National Science Foundation for Young Scientists of China, 2015.1 -2017.12
2. Catalytic synthesis of novel sweetener Rebaudioside A using Pichia pastoris whole cell based on surface display technology, Fundamental Research Funds for the Central Universities, 2017.1 -2018.12
3. Enhancing the production of recombinant proteins in Pichia pastoris by regulating the methanol utilization pathway, Guangdong Province Key Laboratory of Fermentation and Enzyme Engineering Open Fund, 2013.01-2014.12
Representative publications
1. Cheng Li, Ying Lin, Xueyun Zheng, Nuo Pang, Xihao Liao, Xiaoxiao Liu, Yuanyuan Huang, Shuli Liang*, Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris, BMC Biotechnology, 15, 88, 2015
2. Cheng Li, Ying Lin, Yuanyuan Huang, Xiaoxiao Liu, Shuli Liang*, Citrobacter amalonaticus Phytase on the Cell Surface of Pichia pastoris Exhibits High pH Stability as a Promising Potential Feed Supplement, PLOS One, 9, 12, 2014
3. Shuli Liang, Bin Wang, Li Pan, Yanrui Ye, Minghui He, Shuangyan Han, Suiping Zheng, Xiaoning Wang, Ying Lin*, Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing, BMC Genomics, 13, 738, 2012
4. Shuli Liang, Ying Lin, Cheng Li, Yanrui Ye*, Internal ribosome entry site mediates protein synthesis in yeast Pichia pastoris, Biotechnology Letters, 34, 957-964, 2012
5. Shuli Liang, Cheng Li, Yanrui Ye, Ying Lin*, Endogenous signal peptides efficiently mediate the secretion of recombinant proteins in Pichia pastoris, Biotechnology Letters, 35, 97-105, 2013
6. Shuli Liang, Chengjuan Zou, Ying Lin, Xuanwei Zhang, Yanrui Ye*, Identification and characterization of PGCW14: a novel, strong constitutive promoter of Pichia pastoris, Biotechnology Letters, 35, 11, 1865-1871, 2013
7. Li zhang, Shuli Liang, Xinying Zhou, Zi Jin, Fengchun Jiang, Shuangyan Han, Suiping Zheng, Ying Lin*, Screening for glycosylphosphatidylinositol (GPI)-anchored cell wall proteins in Pichia pastoris and their recombinant expression on the cell surface, Applied and Environmental Microbiology, 79, 18, 5519-5526, 2013
8. Zi Jin, Shuli Liang, Xiuqin Zhang, Shuangyan Han, Changqiong Ren, Ying Lin, Suiping Zheng*, Synthesis of Fructose Laurate Esters Catalyzed by a CALB-displaying Pichia pastoris Whole-cell Biocatalyst in a Non-aqueous System, Biotechnology and Bioprocess Engineering, 18, 2, 365-374, 2013
9. Xiaoqiong Lin, Shuli Liang, Shuangyan Han, Suiping Zheng, Yanrui Ye, Ying Lin*, Quantitative iTRAQ LC−MS/MS proteomics reveals the cellular response to heterologous protein overexpression and the regulation of HAC1 in Pichia pastoris, Journal of Proteomics, 91, 58-72,2013
