1. Microbial systems biology, optimization tools for enzyme engineering for pulp and paper industry
In recent years, significant advancements in agriculture, industrial, and biotechnological fields, has fueled the search of microorganisms with novel characteristics that can be utilized in scientific and industrial applications. In this study, we have worked extensively for confirmed combination of enzyme screening and its substrate specificity by using various systems biology approaches in addition to using enzyme modeling tools to originate the implementation of speedy method for industrial screening. The xylanase from T. lanuginous were optimized by using for improved biobleaching of mixed hardwood pulp and process optimization using novel GA-ANN and GA-ANFIS hybrid statistical tools. Several hybrid statistical tools such as Genetic Algorithm-Response Surface Methodology (GA-RSM), Artificial Neural Network (ANN), Genetic Algorithm-Artificial Neural Network (GA-ANN) were employed to obtain more optimized process parameters to maximize the xylanase production. The biobleaching process of mixed hardwood pulp was maximized using OFAT method and various hybrid statistical tools. Indeed, such approaches are supportive in cataloging enzyme processes and its application to diversified areas of microbiology using such approaches.
2. Systems biology for microbes engineering and tools of bioremediation
In recent years, the sustainability is a key factor in terms of any areas of biotechnology. The majority of microbes with their applications for bioethanol, sustainable agriculture, food and feed etc. are not looked upon for their capability enhancement. The current trends in development of technologies like use of systems biology tools, flux balance analysis or modern tools of Artificial intelligence are paving new insights which are very appealing for the researchers. Moreover, there are lots of technologies in these areas, documented and reported but there are many restrictions and their efficiency is also not proven under broader perspectives. Here, in the present work we will throw lights on some of the key aspects of engineered microbes for various applications viz. bioethanol production, plant growth promoting microorganisms (PGPMOs) for sustainable agriculture etc. Here, we present the integrated ideas on use of these techniques in some the above mentioned facets of biotechnology. In addition, we will also present a concise methodology focusing on the use of gene editing techniques, systems biology and metabolic engineering tools for engineering microbe.