我们专注于鉴定与改造介导微生物底盘与功能纳米材料相互作用的关键膜蛋白，旨在优化生物杂合系统在二氧化碳增值转化中的稳定性与效率。我们开发了原位垂钓技术以分析蛋白冠形成的动态过程，借此解析主导生物-非生物识别的特异性表面蛋白。通过这种方法，我们揭示了调控纳米粒子相容性与界面电子转移的分子机制。这些发现为设计新一代抗逆性微生物细胞工厂及自组装生物杂合平台提供了关键科学依据与创新策略。
参考文献 https://pubs.acs.org/doi/10.1021/jacs.4c16987
[en]We focus on identifying and engineering the key membrane proteins that mediate interactions between microbial chassis and functional nanomaterials, aiming to optimize the stability and efficiency of bio-hybrid systems for CO₂ valorization. We developed an in situ 'Fishing' technique to analyze the dynamics of protein corona formation, allowing us to determine the specific surface proteins that govern biotic-abiotic recognition. Through this approach, we uncover the molecular mechanisms regulating nanoparticle compatibility and interfacial electron transfer. These insights provide a critical scientific rationale and new strategies for the design of next-generation, stress-resilient microbial cell factories and self-assembling bio-hybrid platforms.
Refer to J. Am. Chem. Soc., 2025, 147 (9), 7604-7616, https://pubs.acs.org/doi/10.1021/jacs.4c16987 [/en]