报告题目1：

《Enhancing Super Resolution Microscopy - A novel miniaturized incubator system for long-term live-cell imaging》

报告人：Philip Harder

报告题目2：

《Fiber-Optics based Structured Illumination Microscopy to Analyze Fenestrations in Liver Sinusoidal Endothelial Cells》

报告人：Jasmin Schürstedt

报告题目3：

《Improving Stimulated Raman Scattering Microscopy for label-free live cell imaging》

报告人： Luca Schlegel

报告题目4：

《High-Speed Laser Scanning Confocal Microscopy for Deep Tissue Imaging》

报告人：Frederik Niekamp

主持人：韦小明  教授

报告时间：2025年11月12日（星期三）下午15:00

报告地点：物理楼（18号楼）二楼213室学术报告厅

欢迎广大师生参加!

                                     物理与光电学院

                                     2025年11月11日

报告摘要

1. Introducing the fundamentals of incubation and super-resolution microscopy, describe the incubator’s design and development process, and share selected results from preliminary field tests.

2. To study the dynamics and changes of these holes (fenestrations) we need super resolution microscopy. In this presentation, you can have an overview of the working principle of SIM, our fiber SIM setup and the application to murine and human LSECs.

3. The electrical side of SRS microscopy, which is a way of microscopy that allows users to view the composition of their samples without staining. This eliminates many of the negative side effects of traditional microscopy methods used for imaging biological samples and thus has diverse applications in the fields of life science, biology and medicine. Furthermore, this presetation will give some practical tips and pitfalls for people who want to build sensitive and fast optoelectronic front ends.

4. Do you want to image thick biological samples? A conventional confocal microscope can help reduce background and improve contrast — but what if you want to image large volumes, capture thousands of images, or observe fast biological processes? In this presentation, we will introduce you to a novel confocal microscope capable of acquiring confocal images at up to 100 frames per second. This technology opens up new possibilities for high-speed, high-resolution imaging of dynamic biological systems.

 报告人简介

1. Philip Harder, working on Interdisciplinary Biomedicine. He developed a custom miniaturized and lightweight incubator designed for live-cell imaging. The system was built from the ground up to meet the stringent requirements of super-resolution microscopy and to outperform the variability of existing commercial solutions. The incubator provides precise heating, gassing, and medium exchange for long-term imaging and is equipped with integrated sensors on its onboard motherboard to monitor and maintain optimal conditions.

 2. Jasmin Schürstedt-Seher, a PhD student in the Biomolecular Photonics Group of Thomas Huser in Bielefeld University in Germany, working on super resolution fluorescence microscope using structured illumination (SIM), which is based on fiber optical components and capable of 2D and total internal reflection (TIRF) SIM to reduce the phototoxic effect. She is looking at the intracellular holes in liver sinusoidal endothelial cells (LSECs), which have a diameter below the diffraction limit of light (>200 nm).

 3. Luca Schlegel, a PhD student at the Biomolecular Photonics Group of Bielefeld University, working on Stimulated Raman Scattering Microscopy.

 4. Frederik Niekamp, a PhD student in the Biomolecular Photonics Group of Thomas Huser in Bielefeld University in Germany, working on high-speed, high-resolution confocal microscope.