^{Xinpei Li, Qianjie Zhou, Litao Ma, Kun Chen, Panchao Yin*}

    The hybridization of enzymes and inorganics in controlled manner is challenging, however, critical for the development of chemo-enzymatic cascade catalyst with high efficiency and selectivity. Here, proteins and metal oxide clusters can be facilely co-assembled on the surface of colloid of poly(4-vinylpyridine) (P4VP) via hydrogen bonding, due to their enriched surface hydrogen bonding donors. The co-assembly method can be generally applied for preparing chemo-enzymatic catalyst within the selected database of various proteins and metal oxide clusters while the assembly units retain their structures and activities. Typically, a 2.5nm metal oxide cluster {Mo₇₂Fe₃₀}, with peroxidase-like activity, are complexed with glucose oxidase (GOX) on P4VP for the catalysis against the oxidization of o-dianisidine (ODA) with the existence of glucose. Due to the synergistic effects of chemical and enzymatic catalysis, the co-assemblies show even higher ODA oxidation activity compared to GOX/catalase bi-enzymatic system, confirming the effectiveness of the co-assembly protocol for cascade catalysis and enabling its applications in rapid glucose detection and biomass conversion.

    酶与无机物可控杂化对于开发高效、高选择性的化学-酶串联催化剂至关重要，然而其仍然为一项具有挑战性的研究课题。在该项工作中，蛋白质和金属氧化物团簇由于其表面丰富的氢键供体，可通过氢键作用组装在聚4-乙烯基吡啶（P4VP）胶体的表面。所提出的共组装方法广泛应用于选定的蛋白质和金属氧化物团簇，并制备相应的化学酶催化剂，其中组装单元仍保留结构和活性。一种具有过氧化物酶活性的金属氧化物团簇{Mo₇₂Fe₃₀} (尺寸：2.5 nm)，络合P4VP上的葡萄糖氧化酶（GOX），在葡萄糖存在下催化氧化邻二氨基二苯胺（ODA）。相比GOX/过氧化氢酶双酶体系，共组装体系由于化学和酶催化的协同效应，显示出更高催化ODA氧化活性，证实了串联催化共组装方案的有效性，并使其能够应用于快速葡萄糖检测和生物质转化。

文章链接：https://www.sciencedirect.com/science/article/pii/S0021979721003842?via%3Dihub