CZARNA, Anna
✉ al.czarna@gmail.com
Languages: Polish (native); English, German and Russian (fluent); Italian (communicative)
FACULTY ACADEMIC APPOINTMENTS
Institute of Life Sciences, School of Medicine, South China University of Technology
Co-PI (2018-)
JAGIELLONIAN UNIVERSITY, MEDICAL CENTER - UNIVERSITY OF KRAKÓW
Clinical Trials, Methodology, Organization and Management
Kraków, Poland
CARDIOCENTRO TICINO FOUNDATION and SWISS INSTITUTE FOR REGENERATIVE MEDICINE
Research Scientist, 2016 – 2017
Department of Advanced Therapy
Lugano, Switzerland
UIT THE ARCTIC UNIVERSITY TROMSØ - THE ARCTIC UNIVERSITY OF NORWAY
Project management/collaboration, 2015 - 2016
NorStruct - The Norwegian Structural Biology Centre
Tromsø, Norway
UIT THE ARCTIC UNIVERSITY TROMSØ - THE ARCTIC UNIVERSITY OF NORWAY
Postdoctoral fellow, 2015 – 2016
Department of Pharmacy
Tromsø, Norway
UNIVERSITY OF WASHINGTON, SCHOOL OF MEDICINE
International scholarship, invited guest scientist, 2015
Department of Pharmacology
Seattle - WA, USA
Harvard Medical School and Brigham and Women's Hospital
Postdoctoral Fellow, 2012 – 2015
Department of Anesthesiology Perioperative and Pain Medicine – Regenerative Medicine
Boston - MA, USA
Biotec Pharmacon and University of Tromsø
Internship, 2012
Marzymes Project
Tromsø, Norway
Ludwig-Maximilians University of Munich andMax-Planck Institute for Biochemistry
Postdoctoral Fellow, 2009 – 2012
Department of Physiological Chemistry
Adolf Butenandt Institute - Faculty of Medicine
Structural Cell Biology
Munich, Germany
AWARDS & GRANTS
2016 Medical Fellowship on “DUSP2 a novel tumor suppressor in hematological cancer”, Tromsø, Norway.
2002 Scholarship founded by Prof. Pigon for excellent results in exams.
2002 Academic Achievements Scholarship for excellent grades.
LOCAL TEACHING AND TRAINING
Supervision
2016 Supervision of a Master Student in Biochemistry/Cell Biology. University of Tromsø, Norway.
2010 – 2011 Supervision of a Master Student in Biochemistry/ Cell Biology. MPI, Martinsried, Germany.
2007 – 2011 Supervision of Erasmus students from the European student exchange program.
Personal Training
2017 LTK Course Module 1: Introductory Course in Laboratory Animal Science. FELASA accredited course F027/08. Institut für Labortierkunde (LTK) - Institute of Laboratory Animal Science. Zurich, Switzerland.
2015 – 2016 NorStruct - The Norwegian Structural Biology Centre – UiT – official collaboration and work conducted at the crystallography platform, Tromsø, Norway.
2016 MARzymes project coordination: Intership, Biotec Pharmacon and University of Tromsø, Norway.
2012 Klinikum Großhadern, Ludwig-Maximilian Universität, München. Trainee. Kinderherzchirurgie, Herzchirurgische Klinik und Poliklinik, Cardiology and Children Cardiology, Munich, Germany.
2011 CCP4 Study Weekend, University Warwick, Coventry, UK.
2005 Certificate of Teacher Training qualification secondary school and high-school for Biology and chemistry and Environmental Science, Jagiellonian University, Kraków, Poland.
INVITED SPEAKER IN SEMINARS
“International Conference on Wound Care, Tissue Repair and Regenerative Medicine” in London, UK (planned for 14-15.06.2018).
Institute of Molecular Biology GmbH (IMB), Mainz, Germany (12.12.2017).
School of Food Science and Technology, South China University of Technology, Tianhe, Guangzhou, Guangdong, P. R. China (23.02.2018 and 09.03.2018).
“MCB Diamond Seminar” at Małopolska Centre of Biotechnology of the Jagiellonian University (MCB/JU), Krakow, Poland, (16.01.2018).
European Molecular Biology Laboratory, Heidelberg, Germany (12.12.2017).
Stem Cell Unit, Herzzentrum Göttingen - Universitätsmedizin Göttingen, Germany (26.09.2017).
Department of Pharmacology, School of Medicine, University of Washington, Seattle WA, USA, (07.07.2015).
EDUCATION
2017 Extramural study in Clinical Trials Methodology organization and management.
GCP certificate, Post-diploma Medical Training Center, Jagiellonian University, Kraków, Poland.
2006 – 2009 Ph.D. in Biochemistry/Chemistry.
Technische Universität München, MPI Biochemie, Munich, Germany.
Prof. N. Budisa and Prof. T. Holak
2003 – 2006 Pedagogical Study, Jagiellonian University, Kraków, Poland.
Block of psychological-pedagogical and didactic classes.
Prof. J. Godula
2001 – 2006 M.Sc. in Biology, Biochemistry specialization.
Jagiellonian University Faculty of Biology and Earth Science, Kraków, Poland.
Prof. A. Dubin
PEER-REVIEWED PUBLICATIONS
JOURNAL ARTICLES
Xinyu He, Song Shen, Cong-Fei Xu, Czarna A, Jun Wang. Delivery of siRNA for Therapeutic Application. (2018 Dec; Submitted to Cancer Biology & Therapy)
Czarna A et al. The mouse lung is regulated by a compartment of stem cells. Manuscript. Ready for submission, 2018.
Czarna A et al. Lung stem cells and chronic obstructive pulmonary disease in humans. Manuscript. Ready for submission, 2018.
Matsuda A, Sakairi Y, Czarna A et al,. Progressive pulmonary fibrosis in humans is a stem cell disease. Manuscript. Ready for submission, 2018.
Wang F, Zhang H, Chen W, Czarna A, Yang B, Wang Y. Function of C-terminal peptides on enzymatic and interfacial adsorption properties of lipase from Gibberella zeae. Biochim Biophys Acta Gen Subj. 2018 Dec;1862(12):2623-2631.
Czarna A et al. Novel scaffolds for DYRK1A inhibition. J Med Chem. 2018 Sep 13;61(17):7560-7572.
Czarna A, Sanada F, Matsuda A, Kim J, Signore S, Pereira JD, Sorrentino A, Kannappan R, Cannata A, Hosoda T, Rota M, Crea F, Anversa P, Leri A. Single-cell analysis of the fate of c-kit-positive bone marrow cells. NPJ Regenerative Medicine. 2017;2:27. Corresponding author.
Kannappan R, Matsuda A, Ferreira-Martins J, Zhang E, Palano G, Czarna A, Cabral-Da-Silva MC, Bastos-Carvalho A, Sanada F, Ide N, Rota M, Blasco MA, Serrano M, Anversa P, Leri A. P53 modulates the fate of cardiac progenitor cells ex vivo and in the diabetic heart in vivo. EBioMedicine. 2017;16:224-237.
Pustelny K, Zdzalik M, Stach N, Stec-Niemczyk J, Cichon P, Czarna A, Popowicz G, Mak P, Drag M, Salvesen GS, Wladyka B, Potempa J, Dubin A, Dubin G. Staphylococcal splb serine protease utilizes a novel molecular mechanism of activation. The Journal of biological chemistry. 2014;289:15544-15553.
Sanada F, Kim J, Czarna A, Chan NY, Signore S, Ogorek B, Isobe K, Wybieralska E, Borghetti G, Pesapane A, Sorrentino A, Mangano E, Cappetta D, Mangiaracina C, Ricciardi M, Cimini M, Ifedigbo E, Perrella MA, Goichberg P, Choi AM, Kajstura J, Hosoda T, Rota M, Anversa P, Leri A. C-kit-positive cardiac stem cells nested in hypoxic niches are activated by stem cell factor reversing the aging myopathy. Circulation research. 2014;114:41-55.
Czarna A, Berndt A, Singh HR, Grudziecki A, Ladurner AG, Timinszky G, Kramer A, Wolf E. Structures of drosophila cryptochrome and mouse cryptochrome1 provide insight into circadian function. Cell. 2013;153:1394-1405.
Czarna A, Breitkreuz H, Mahrenholz CC, Arens J, Strauss HM, Wolf E. Quantitative analyses of cryptochrome-mbmal1 interactions: Mechanistic insights into the transcriptional regulation of the mammalian circadian clock. The Journal of biological chemistry. 2011;286:22414-22425.
Hulme C, Akritopoulou-Zanze I, Dai WM, Beck B, Srivastava S, Wang W, Wang K, Czarna A, Holak TA and Meireles L, et al. Multi-Component Reactions in Drug Discovery. Advances in Experimental Medicine and Biology. 2011, Volume 699, 75-106.
Popowicz GM, Czarna A, Wolf S, Wang K, Wang W, Dömling A, Holak TA. Structures of low molecular weight inhibitors bound to MDMX and MDM2 reveal new approaches for p53-MDMX/MDM2 antagonist drug discovery. Cell Cycle. 2010;9:1104–1111.
Srivastava S, Beck B, Wang W, Czarna A, Holak TA, Domling A. Rapid and efficient hydrophilicity tuning of p53/mdm2 antagonists. Journal of combinatorial chemistry. 2009;11:631-639.
Czarna A, Beck B, Srivastava S, Popowicz GM, Wolf S, Huang Y, Bista M, Holak TA, Domling A. Robust generation of lead compounds for protein-protein interactions by computational and mcr chemistry: P53/hdm2 antagonists. Angewandte Chemie. 2010;49:5352-5356.
Czarna A, Popowicz GM, Pecak A, Wolf S, Dubin G, Holak TA. High affinity interaction of the p53 peptide-analogue with human mdm2 and mdmx. Cell cycle. 2009;8:1176-1184.
Popowicz GM, Czarna A, Holak TA. Structure of the human mdmx protein bound to the p53 tumor suppressor transactivation domain. Cell cycle. 2008;7:2441-2443.
Popowicz GM, Czarna A, Rothweiler U, Szwagierczak A, Krajewski M, Weber L, Holak TA. Molecular basis for the inhibition of p53 by mdmx. Cell cycle. 2007;6:2386-2392.
Rothweiler U, Czarna A, Krajewski M, Ciombor J, Kalinski C, Khazak V, Ross G, Skobeleva N, Weber L, Holak TA. Isoquinolin-1-one inhibitors of the mdm2-p53 interaction. ChemMedChem. 2008;3:1118-1128.
Rothweiler U, Czarna A, Weber L, Popowicz GM, Brongel K, Kowalska K, Orth M, Stemmann O, Holak TA. NMR screening for lead compounds using tryptophan-mutated proteins. J Med Chem 2008. 51: 5035-5042.
Dubin G, Stec-Niemczyk J, Kisielewska M, Pustelny K, Popowicz GM, Bista M, Kantyka T, Boulware KT, Stennicke HR, Czarna A, Phopaisarn M, Daugherty PS, Thogersen IB, Enghild JJ, Thornberry N, Dubin A, Potempa J. Enzymatic activity of the staphylococcus aureus splb serine protease is induced by substrates containing the sequence trp-glu-leu-gln. Journal of molecular biology. 2008;379:343-356.
Popowicz GM, Dubin G, Stec-Niemczyk J, Czarna A, Dubin A, Potempa J, Holak TA. Functional and structural characterization of spl proteases from staphylococcus aureus. Journal of molecular biology. 2006;358:270-279.
CONFERENCE PROCEEDINGS
American Heart Association Scientific Sessions, AHA; November 12-16, 2016. New Orleans, Louisiana, USA:
Lung Stem Cells and Chronic Obstructive Pulmonary Disease; Czarna A, Sakairi Y, Matsuda A, Hatzistergos KE, Liu X, Luciani M, Graciotti L, Di Rocco A, Hosoda T, Bueno R, Camp PC, Rota M, Saur D, Seidler B, Yoshino I, Mocetti M, Moccetti T, Hare JM, Perrella MA, Rosas IO, Leri A, Anversa P - poster presentation.
Retrospective 14C Birth Dating of Human Cardiomyocytes; Matsuda A, Czarna A, Kim J, Moccetti M, Hosoda T, Rota M, Leri A, Moccetti T, Anversa P - poster presentation.
Single-Cell analysis of the Fate of c-kit-Positive Bone Marrow Cells; Czarna A, Sanada F, Matsuda A, Kim J, Signore S, Sorrentino A, Kannappan R, Hosoda T, Moccetti T, Anversa P, Rota M, Leri A - poster presentation
Research Education Event; Cardiocentro Ticino Foundation, University of Zurich, Swiss Institute for Regenerative Medicine (SIRM); November 3rd, 2016, Lugano, Switzerland.
“Cell Analysis of the Fate of c-kit-Positive Bone Marrow Cells” - oral presentation
Rebuilding the Failing Heart, annual symposium; May 9-10th, 2016. Cardiocentro Ticino, Swiss Institute for Regenerative Medicine (SIRM) Lugano, Switzerland – invited guest.
Basic Cardiovascular Sciences BCVS; July 14-29, 2014. Las Vegas, Nevada, USA:
Bone Marrow Cells Transdifferentiate into Cardiomyocytes and Repair the Infarcted Heart; Czarna A, Kim J, Sanada F, Signore S, Cannata A, Rota M, Leri A, Anversa P - poster presentation.
Molecular Signature Of Cardiomyogenic c-kit-positive Bone Marrow Cells; Czarna A, Sanada F, Pereira JD, Churko J, Wu JC, Rota M, Anversa P, Leri A - poster presentation.
American Heart Association Scientific Sessions, AHA; November 16-20, 2013 Dallas, Texas, USA:
Genetic Ablation of c-kit Impairs Cardiac Stem Cell Function, Leading to Attenuated Cardiomyogenesis and Accelerated Myocardial Aging; Czarna A, Sanada F, Kim J, Signore S, Goichberg P, Kajstura J, Rota M, Anversa P, Leri A - poster presentation.
Stem Cell Factor Activates Hypoxic Cardiac Stem Cells Improving Myocardial Aging; Sanada F, Kim J, Czarna A, Signore S, Anversa P, Leri A - poster presentation.
Basic Cardiovascular Sciences BCVS; July 22-25, 2013 Las Vegas, Nevada, USA:
Hypoxic and Normoxic Niches Regulate Cardiac Stem Cell Fuction; Sanada F, Kim J, Czarna A, Signore S, Sorrentino A, Mangiaracina C, Kannappan R, Goichberg P, Rota M, Hosoda T, Kajstura J, Anversa P, Leri A - poster presentation.
Clonal C-kit-Positive Bone Marrow Cells and Cardiomyogenesis; Sanada F, KimJ, Czarna A, Signore S, Sorrentino A, Mangiaracina A, Kannappan R, Goichberg P, Rota M, Hosoda T, Kajstura J, Anversa P, Leri A - poster presentation.
G R C Chronobiology; June 12-17, 2011, Lucca (Barga), Italy - poster presentation.
Blue Light Photoreceptors; Frauenwoerth/Chiemse; 14-17 March, 2010 - oral presentation.
Frontiers of NMR in Biology; Keystone Symposium organized by Ikura M, McDermott AE and Bax A; February 15-20, 2009, Santa Fe, New Mexico, USA - poster presentation.
Advances in NMR of protein and nucleic acid molecular recognition; Conference in the framework of the EU NMR-Life Coordination Action project; Oct 16-18, 2008, Murnau, Germany - oral presentation.
Magnetic Resonance in Biology and Chemistry; (Germany-Switzerland-Austria), GDCh Fachgruppe Magnetische Resonanzspektroskopie, Sep 22-25, 2008, in Regensburg, Germany - oral presentation.
XXXIII Winterschool for Biochemistry, Biophysics and Biotechnology UJ, 25-29 Feb 2006, Krynica – oral presentation.