(Lecture, June 18) MALDI mass spectrometry imaging study

Title：MALDI mass spectrometry imaging study

Time: June 18, 2024 (Tuesday) 11:00 am - 12:00 am

Venue: Conference Room 105, Shaw Engineering Building

[Biography]

Nie Zongxiu is a researcher at the Institute of Chemistry, Chinese Academy of Sciences and a recipient of the National Outstanding Youth Fund. The main research direction is the construction of particle mass spectrum and the mass spectrum of small molecule metabolism. A variety of particle ionization sources and charge detectors were designed and developed, and an ion trap particle mass spectrometer platform was constructed to characterize micro-nano particles with multiple parameters. The particle analysis method based on fingerprint mass spectrometry was established, and the biological tissue mass spectrometry image of nanoparticles was obtained, which widened the mass spectrum from 10e6 Da of molecular mass spectrometry to 10e14Da of particles by 8 orders of magnitude. A series of new MALDI substrates have been synthesized and discovered, and small molecule mass spectrometry images of metabolism in various animal models have been obtained. In recent years, in Nature Nanotech., Science Advance, Angew. Chem., Chem. Soc. Rev., Anal. Chem. He has published more than 130 papers in other journals. Work published in Nature Nanotech., reviewed by journal articles in the accompanying "News Perspective," was selected for Nature Nanotech 2016. The magazine's 10th anniversary issue. Granted 32 Chinese invention patents.

[Abstract]

Matrix assisted laser desorption ionization (MALDI) mass spectrometry has become an indispensable tool in proteomics, genomics, metabolomics, glycosomics, lipidomics, and mass spectroscopic imaging. MALDI's mass spectrometry of small molecule compounds (m/z < 1000) is hampered by the severe background interference of traditional substrates in the small molecule region. In recent years, we have been committed to the study of substrates without background interference for mass spectrometry analysis and imaging of small and medium molecular metabolites in complex biological systems, and developed and synthesized a series of new organic salt substrates with high salt tolerance and low background interference. In combination with animal models, mass spectrometry imaging of biological tissue slices was realized, and the metabolic changes and spatial distribution of non-lipid small molecule compounds and glycerophospholipids were detected in situ. In addition, the distribution of carbon nanomaterials in biological tissues and tissue suborgans was obtained based on the characteristic fingerprint mass spectrometry of the low molecular weight region of nanomaterials. It was found that carbon nanotubes and carbon nanodots were mainly distributed in the outer parenchyma of kidney tissue, and all materials were distributed in the red pulp of spleen, with the highest concentration of carbon nanotubes in the marginal region of spleen tissue. Recently, the method has been applied to drug release in nanoborne drug systems by quantitatively monitoring the inherent mass spectrometry signal intensity ratio between nanocarriers and drug molecules, quantitatively analyzing and visualizing the in situ release of nanocarriers in tissues and the differences in the distribution of organic carbon and elemental carbon in carbonaceous aerosols.

Announced by School of Chemistry and Chemical Engineering