Huafeng Xie, Ph.D.

PI

Huafeng Xie, Ph.D, Professor

Laboratory of Stem cells and Epigenetics

E-mail:xiehuafeng@scut.edu.cn

Education

Ph.D. (2000-2005)   Albert Einstein College of Medicine

B.S.    (2002-2006)   Polymer Chemistry and Biomaterials,University of Science and Technology of China

Working Experience

Principal Investigator (2018-present) School of Medicine/ Institutes for Life Science, SCUT
Lecturer (2014-2017)    Harvard Medical School, Harvard University
Postdoctoral Fellow (2006-2013) 

Dana-Farber Cancer Institute, Harvard Medical School, Harvard 

University

Postdoctoral Fellow (2006-2006) Albert Einstein College of Medicine

Research Interests

Research epigenetics in stem cell self-replication, somatic differentiation, cell reprogramming, and regulation in leukemia cells by using gnetics, molecular biology and omics as the main research methods.Through the modification of the relevant epigenetic and intervention of the signaling pathways to achieve: 1) Pluripotent stem cell vitro directed differentiation to produce hematopoietic stem cells and platelets; 2) Hematopoietic stem cell expansion in vitro; 3) Enhances cell plasticity and promotes cell reprogramming. Meanwhile, finding new therapeutic targets for leukemia stem cells by genetic screening.

Research Achievements

For the first time, inducing primary cells to achieve cell reprogramming with transcription factors undisputed proved that the terminally differentiated cells still retain the cell plasticity.It laid the foundation for reprogramming of iPS cells. Researches revealed the regulatory mechanism of epigenetic modification on the self-replication and differentiation of hematopoietic stem cells, and the specific dependence of leukemia stem cells on the epigenetic gene EZH2, provided a new therapeutic target and thinking for the treatment of related leukemia. The results published in top international academic journals, such as Cell, Nature, Cell StemCell and Cancer Discovery etc. the articles have been cited about 2500 times.

Representative Publications

1. H. Xie, C. Peng, J. Huang, B. E. Li, W. Kim, E. C. Smith, Y. Fujiwara, J. Qi, G. Cheloni, P. P. Das, M. Nguyen, S. Li, J. E. Bradner, S. H. Orkin, Chronic Myelogenous Leukemia- Initiating Cells Require Polycomb Group Protein EZH2. Cancer discovery6, 1237-1247 (2016)

2. H. Xie, J. Xu, J. H. Hsu, M. Nguyen, Y. Fujiwara, C. Peng, S. H. Orkin, Polycomb repressive complex 2 regulates normal hematopoietic stem cell function in a developmental-stage-specific manner. Cell stem cell 14, 68-80 (2014)

3. H. Xie, C. V. Laiosa, T. Graf, Reprogramming of committed lymphoid cells by enforced transcription factor expression. Methods in molecular biology 636, 219-232

4. H. Xie, S. H. Orkin, Immunology: changed destiny. Nature 449, 410-411 (2007)

5. H. Xie, M. Ye, R. Feng, T. Graf, Stepwise reprogramming of B cells into macrophages. Cell 117, 663-676 (2004)

6. M. A. Erb, T. G. Scott, B. E. Li, H. Xie, J. Paulk, H. S. Seo, A. Souza, J. M. Roberts, S. Dastjerdi, D. L. Buckley, N. E. Sanjana, O. Shalem, B. Nabet, R. Zeid, N. K. Offei-Addo, S. Dhe-Paganon, F. Zhang, S. H. Orkin, G. E. Winter, J. E. Bradner, Transcription control by the ENL YEATS domain in acute leukaemia. Nature 543, 270-274 (2017)

7. S. Ai, Y. Peng, C. Li, F. Gu, X. Yu, Y. Yue, Q. Ma, J. Chen, Z. Lin, P. Zhou, H. Xie, T. W. Prendiville, W. Zheng, Y. Liu, S. H. Orkin, D. Z. Wang, J. Yu, W. T. Pu, A. He, EED orchestration of heart maturation through interaction with HDACs is H3K27me3-independent. eLife 6,(2017)

8. M. Serresi, G. Gargiulo, N. Proost, B. Siteur, M. Cesaroni, M. Koppens, H. Xie, K. D. Sutherland, D. Hulsman, E. Citterio, S. Orkin, A. Berns, M. van Lohuizen, Polycomb Repressive Complex 2 Is a Barrier to KRAS-Driven Inflammation and Epithelial-Mesenchymal Transition in Non-Small-Cell Lung Cancer. Cancer cell 29, 17-31 (2016)

9. F. Mirzamohammadi, G. Papaioannou, J. B. Inloes, E. B. Rankin, H. Xie, E. Schipani, S. H. Orkin, T. Kobayashi, Polycomb repressive complex 2 regulates skeletal growth by suppressing Wnt and TGF-beta signalling. Nature communications 7, 12047 (2016)

10. E. Danis, T. Yamauchi, K. Echanique, X. Zhang, J. N. Haladyna, S. S. Riedel, N. Zhu, H. Xie, S. H. Orkin, S. A. Armstrong, K. M. Bernt, T. Neff, Ezh2 Controls an Early Hematopoietic Program and Growth and Survival Signaling in Early T Cell Precursor Acute Lymphoblastic Leukemia. Cell reports 14, 1953-1965 (2016)

11. J. Yin, J. W. Leavenworth, Y. Li, Q. Luo, H. Xie, X. Liu, S. Huang, H. Yan, Z. Fu, L. Y. Zhang, L. Zhang, J. Hao, X. Wu, X. Deng, C. W. Roberts, S. H. Orkin, H. Cantor, X. Wang, Ezh2 regulates differentiation and function of natural killer cells through histone methyltransferase activity. Proceedings of the National Academy of Sciences of the United States of America 112, 15988-15993 (2015)

12. J. Xu, Z. Shao, D. Li, H. Xie, W. Kim, J. Huang, J. E. Taylor, L. Pinello, K. Glass, J. D. Jaffe, G. C. Yuan, S. H. Orkin, Developmental control of polycomb subunit composition by GATA factors mediates a switch to non-canonical functions. Molecular cell 57, 304-316 (2015)

13. P. P. Das, D. A. Hendrix, E. Apostolou, A. H. Buchner, M. C. Canver, S. Beyaz, D. Ljuboja, R. Kuintzle, W. Kim, R. Karnik, Z. Shao, H. Xie, J. Xu, A. De Los Angeles, Y. Zhang, J. Choe, D. L. Jun, X. Shen, R. I. Gregory, G. Q. Daley, A. Meissner, M. Kellis, K. Hochedlinger, J. Kim, S. H. Orkin, PRC2 Is Required to Maintain Expression of the Maternal Gtl2-Rian-Mirg Locus by Preventing De Novo DNA Methylation in Mouse Embryonic Stem Cells. Cell reports 12, 1456-1470 (2015)

14. T. Neff, A. U. Sinha, M. J. Kluk, N. Zhu, M. H. Khattab, L. Stein, H. Xie, S. H. Orkin, S. A. Armstrong, Polycomb repressive complex 2 is required for MLL-AF9 leukemia. Proceedings of the National Academy of Sciences of the United States of America 109, 5028-5033 (2012)

15. A. He, Q. Ma, J. Cao, A. von Gise, P. Zhou, H. Xie, B. Zhang, M. Hsing, D. C. Christodoulou, P. Cahan, G. Q. Daley, S. W. Kong, S. H. Orkin, C. E. Seidman, J. G. Seidman, W. T. Pu, Polycomb repressive complex 2 regulates normal development of the mouse heart. Circulation research 110, 406-415 (2012)

16. M. Yu, L. Riva, H. Xie, Y. Schindler, T. B. Moran, Y. Cheng, D. Yu, R. Hardison, M. J. Weiss, S. H. Orkin, B. E. Bernstein, E. Fraenkel, A. B. Cantor, Insights into GATA-1-mediated gene activation versus repression via genome-wide chromatin occupancy analysis. Molecular cell 36, 682-695 (2009)

17. R. Feng, S. C. Desbordes, H. Xie, E. S. Tillo, F. Pixley, E. R. Stanley, T. Graf, PU.1 and C/EBPalpha/beta convert fibroblasts into macrophage-like cells. Proceedings of the National Academy of Sciences of the United States of America 105, 6057-6062 (2008)

18. C. V. Laiosa, M. Stadtfeld, H. Xie, L. de Andres-Aguayo, T. Graf, Reprogramming of committed T cell progenitors to macrophages and dendritic cells by C/EBP alpha and PU.1 transcription factors. Immunity 25, 731-744 (2006)

19. M. Ye, H. Iwasaki, C. V. Laiosa, M. Stadtfeld, H. Xie, S. Heck, B. Clausen, K. Akashi, T. Graf, Hematopoietic stem cells expressing the myeloid lysozyme gene retain long-term, multilineage repopulation potential. Immunity 19, 689-699 (2003)

Address: B2 Building, South China University of Technology,382 Waihuandong Road, Higher Education Mega Center, Guangzhou, Guangdong 510006, China
Tel: 020-39381182