Research scholar shared scientific research on peptide and protein in SCUT
time: 2017-06-22

A lecture entitled “One - Pot N2C / C2C / N2N Ligation To Trap Weak Protein -Protein Interactions” was given by Dr. Zhao Lei, director of Max Planck Institute of Molecular Physiology for teachers and students of the School of Biology and Biological Engineering on April 10.

During the lecture, Dr. Zhao shared his research in the Institute of Molecular Physiology in Germany with regard to peptides and proteins. He pointed out that protein-protein interaction plays an essential role in the life process. And weak protein-protein interaction has the characteristics of short duration of action and fast dissociation rate, which is the key link in cell signal transmission. However, such weak interactions pose a challenge for obtaining stable protein complexes, which presents considerable difficulty in studying weak protein-protein interactions.

A protein-protein weak interaction could increase the interaction probability of the termini from both proteins, thereby facilitating chemical protein ligation and trapping the transient protein complexes in an unbiased manner. Statistically, there will be four possibilities to link two proteins at termini. To solve the difficulty, Dr. Zhao presented a general strategy to trap weak protein–protein interactions using N-terminal to N-terminal (N2N) ligation and protein C-terminal to C-terminal (C2C) ligation except for using the traditional protein amino terminal and carboxyl terminal (N2C) connection method. His team carried out all reactions in one pot to determine whether the ligation strategies could efficiently trap the weak protein-protein interaction and facilely identify the most favorable one. This method can quickly and unequivocally obtain protein complexes, which opens up a new avenue to investigate protein interactions and the associated biological processes.